Supplemental Data In Vivo Fluorescent Detection of Fe - S Clusters Coordinated by Human GRX 2
نویسندگان
چکیده
Analytical gel filtration A Superdex 75 column driven by an AKTA FPLC system (GE Healthcare) was used to separate monomeric and dimeric Venus-GRX2 immediately prior to spectral measurements and to evaluate the extent to which Venus-Gcn4 had been converted to monomer by enterokinase. All gel filtration experiments involving these proteins were performed using columns that had been equilibrated in PSG buffer. The apparent molecular weights of the eluted proteins were estimated using a standard curve generated with BSA (66 kDa), carbonic anhydrase (29 kDa), and cytochrome C (12.4 kDa).
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